PrimePCR™ SYBR® Green Assay: Hbq1b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0032039

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hbq1b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hbq1b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0001656
Assay Design:   Exonic
Chromosome Location:   11:32287600-32287720question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000101387

Gene Information

This gene is one of two mouse theta-globin genes found in the alpha-globin gene cluster on chromosome 11. This gene represents the T1 (or 5') theta-globin gene described in PMIDs 18245844 and 11157800 respectively. [provided by RefSeq Apr 2009]

Gene Symbol:   Hbq1b
Gene Name:   Hemoglobin, theta 1B
Aliases:   F830116E18Rik, Hbqt1
RefSeq:   NC_000077.6 NT_039515.7
Ensembl:   ENSMUSG00000073063
Entrez:   544763
Chromosome Mapping:   11 A4|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.890000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download