This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0032007
PrimePCR™ PreAmp for SYBR® Green Assay: Npw, Mouse
PrimePCR™ Template for SYBR® Green Assay: Npw, Mouse
Orthologs of this gene express a preproprotein that is processed into a neuropeptide ligand for two G-protein coupled receptors in the central nervous system. The neuropeptide is thought to directly or indirectly regulate feeding weight regulation and pain response. The preproprotein is translated using a non-AUG initiation codon. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.