PrimePCR™ SYBR® Green Assay: Gpx4, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0001062
Assay Design:   Exonic
Chromosome Location:   10:80054514-80054724question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000105372 ENSMUST00000097227

Gene Information

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide organic hydroperoxide and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation rat produces proteins that localize to the nucleus mitochondrion and cytoplasm. In mice experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Two pseudogenes exist for this gene one on chromosome 10 and one on chromosome 17. [provided by RefSeq Jul 2008]

Gene Symbol:   Gpx4
Gene Name:   Glutathione peroxidase 4
Aliases:   1700027O09Rik, PHGPx, mtPHGPx, snGPx
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000075706
Entrez:   625249
UniGene:   Mm.359573
Chromosome Mapping:   10 C1|10 43.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.900000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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