PrimePCR™ SYBR® Green Assay: GUSB, Rhesus Monkey

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GUSB, Rhesus Monkey
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GUSB, Rhesus Monkey
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMccCID0011964
Assay Design:   Intron-spanning
Chromosome Location:   3:59000702-59004394question
Amplicon Length:   102
Splice Variants Targeted:   ENSMMUT00000029878

Gene Information

The human homolog of this gene, GUSB, encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. In human, the enzyme forms a homotetramer that is localized to the lysosome. Mutations in the human gene result in mucopolysaccharidosis type VII. There are many pseudogenes of this locus in the human genome. Compared to human, the rhesus macaque genome has a segmental duplication of this locus and may have two functional GUSB genes (GUSBa and GUSBb). However the protein coding status of the rhesus GUSBa and GUSBb genes remains provisional since there are small gaps in the Mmul_051212 assembly at these two loci. ESTs from other macaque species indicate that some macaque species can produce full-length GUSB transcripts. [provided by RefSeq, Oct 2010]

Gene Symbol:   GUSB
Gene Name:   Glucuronidase, beta
Aliases:   Not Available
RefSeq:   XM_001087699
Ensembl:   ENSMMUG00000021236
Entrez:   677692
Chromosome Mapping:   3

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download