This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: GUSB, Rhesus Monkey
PrimePCR™ Template for SYBR® Green Assay: GUSB, Rhesus Monkey
The human homolog of this gene, GUSB, encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. In human, the enzyme forms a homotetramer that is localized to the lysosome. Mutations in the human gene result in mucopolysaccharidosis type VII. There are many pseudogenes of this locus in the human genome. Compared to human, the rhesus macaque genome has a segmental duplication of this locus and may have two functional GUSB genes (GUSBa and GUSBb). However the protein coding status of the rhesus GUSBa and GUSBb genes remains provisional since there are small gaps in the Mmul_051212 assembly at these two loci. ESTs from other macaque species indicate that some macaque species can produce full-length GUSB transcripts. [provided by RefSeq, Oct 2010]