PrimePCR™ lncRNA® Probe Assay:TAF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLID0228633

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: TAF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: TAF4, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLIP0241891
Assay Design:   intron_spanning
Chromosome Location:   chr20:61953700-61997612question
Amplicon Length:   162
Splice Variants Targeted:   ENST00000474089
LNCipedia Splice Variants Targeted:   lnc-TAF4-1:1

Gene Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) which binds to the core promoter to position the polymerase properly serves as the scaffold for assembly of the remainder of the transcription complex and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription serve as coactivators function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid thyroid hormone and vitamin D3 receptors. In addition this subunit interacts with the transcription factor CREB which has a glutamine-rich activation domain and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq Jul 2008]

Gene Symbol:   TAF4
Gene Name:   TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
Aliases:   FLJ41943, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000130699
LNCipedia:   lnc-TAF4-1
Entrez:   6874
Chromosome Mapping:   20q13.33
Gene Strand:   Not Available

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