PrimePCR™ lncRNA SYBR® Green Assay:EGLN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0211537

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: EGLN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: EGLN1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0134297
Assay Design:   intron_spanning
Chromosome Location:   chr1:231370614-231374029question
Amplicon Length:   104
Refseq Transcripts Amplified:   NM_022051,XM_005273166
Splice Variants Targeted:   ENST00000476717 ENST00000366641
LNCipedia Splice Variants Targeted:   lnc-EXOC8-3:1

Gene Information

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor and under normal oxygen concentration modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq Nov 2009]

Gene Symbol:   EGLN1
Gene Name:   egl nine homolog 1 (C. elegans)
Aliases:   C1orf12, DKFZp761F179, ECYT3, HIFPH2, HPH2, PHD2, SM20, ZMYND6
RefSeq:   NC_000001.10 NG_015865.1 NT_167186.1
Ensembl:   ENSG00000135766
LNCipedia:   lnc-EXOC8-3
Entrez:   54583
Chromosome Mapping:   1q42.1
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download