PrimePCR™ lncRNA SYBR® Green Assay:NFU1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0210726

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: NFU1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: NFU1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0133486
Assay Design:   intron_spanning
Chromosome Location:   chr2:69406027-69415239question
Amplicon Length:   81
Refseq Transcripts Amplified:   NM_001002755,NM_001002756,NM_015700,XM_017003808,NR_045632,NR_045631
Splice Variants Targeted:   ENST00000419370 ENST00000410022 ENST00000303698 ENST00000462320 ENST00000474230 ENST00000450796 ENST00000471185 ENST00000484177 ENST00000394305
LNCipedia Splice Variants Targeted:   lnc-NFU1-1:2

Gene Information

This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1 and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   NFU1
Gene Name:   NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
Aliases:   HIRIP5, MGC142252, MGC142254, NIFUC, Nfu, NifU
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000169599
LNCipedia:   lnc-NFU1-1
Entrez:   27247
Chromosome Mapping:   2p15-p13
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download