PrimePCR™ lncRNA SYBR® Green Assay:GJB6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0181754

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: GJB6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: GJB6, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0104514
Assay Design:   intron_spanning
Chromosome Location:   chr13:20223415-20229624question
Amplicon Length:   95
Refseq Transcripts Amplified:   NM_001110221,NM_001110220,NM_001110219,XM_017020359,XM_017020360,XM_017020358,XM_017020357,NM_006783
Splice Variants Targeted:   ENST00000356192 ENST00000400065 ENST00000400066 ENST00000241124
LNCipedia Splice Variants Targeted:   lnc-GJB2-2:3 lnc-GJB2-2:2 lnc-GJB2-2:1

Gene Information

Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments two extracellular loops a cytoplasmic loop formed between the two inner transmembrane segments and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past connexin protein names were based on their molecular weight however the new nomenclature uses sequential numbers based on which form (alpha or beta) of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia. [provided by RefSeq Jul 2008]

Gene Symbol:   GJB6
Gene Name:   gap junction protein, beta 6, 30kDa
Aliases:   CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, HED
RefSeq:   NC_000013.10 NT_024524.14 NG_008323.1
Ensembl:   ENSG00000121742
LNCipedia:   lnc-GJB2-2
Entrez:   10804
Chromosome Mapping:   13q11-q12.1,13q12
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download