PrimePCR™ lncRNA SYBR® Green Assay:CRYBB3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0180926

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: CRYBB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: CRYBB3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0103686
Assay Design:   intron_spanning
Chromosome Location:   chr22:25201466-25203858question
Amplicon Length:   189
Refseq Transcripts Amplified:   XM_017028599,XM_011529902,NM_004076
Splice Variants Targeted:   ENST00000215855 ENST00000404334
LNCipedia Splice Variants Targeted:   lnc-KIAA1671-1:1

Gene Information

Crystallins are separated into two classes: taxon-specific or enzyme and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development these crystallins are made and then retained throughout life making them extremely stable proteins. Mammalian lens crystallins are divided into alpha beta and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs a connecting peptide and N- and C-terminal extensions. Beta-crystallins the most heterogeneous differ by the presence of the C-terminal extension (present in the basic group none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene a beta basic group member is part of a gene cluster with beta-A4 beta-B1 and beta-B2. [provided by RefSeq Jul 2008]

Gene Symbol:   CRYBB3
Gene Name:   crystallin, beta B3
Aliases:   CATCN2, CRYB3, MGC125772, MGC125773, MGC125774
RefSeq:   NC_000022.10 NG_009828.1 NT_011520.12
Ensembl:   ENSG00000100053
LNCipedia:   lnc-KIAA1671-1
Entrez:   1417
Chromosome Mapping:   22q11.2-q12.1,22q11.23
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download