PrimePCR™ lncRNA SYBR® Green Assay:SPG20-AS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0147041

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0069801
Assay Design:   intron_spanning
Chromosome Location:   chr13:36365775-36368233question
Amplicon Length:   75
Refseq Transcripts Amplified:   NR_045180,NR_045181
Splice Variants Targeted:   ENST00000493739 ENST00000379848 ENST00000488319
LNCipedia Splice Variants Targeted:   SPG20-AS1:3 SPG20-AS1:1 SPG20-AS1:2 SPG20-AS1:5 SPG20-AS1:4

Gene Information

Not Available

Gene Symbol:   SPG20-AS1
Gene Name:   SPG20 antisense RNA 1 [Source:HGNC Symbol;Acc:HGNC:39933]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000120664
LNCipedia:   SPG20-AS1
UniGene:   Not Available
Chromosome Mapping:   Not Available
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.830002
Efficiency 1.98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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