PrimePCR™ lncRNA® Probe Assay:ARFGAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0216266

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: ARFGAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: ARFGAP1, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0229524
Assay Design:   exonic
Chromosome Location:   chr20:63285843-63285949question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000547204 ENST00000549047 ENST00000523460 ENST00000520485 ENST00000518601 ENST00000518794 ENST00000518691 ENST00000395285 ENST00000518618 ENST00000520022 ENST00000468975 ENST00000353546 ENST00000370275 ENST00000519273 ENST00000370283 ENST00000519604
LNCipedia Splice Variants Targeted:   lnc-ARFGAP1-4:6 lnc-ARFGAP1-4:5 lnc-ARFGAP1-4:3 lnc-ARFGAP1-4:1 lnc-ARFGAP1-4:2 lnc-ARFGAP1-4:4

Gene Information

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ARFGAP1
Gene Name:   ADP-ribosylation factor GTPase activating protein 1
Aliases:   ARF1GAP, HRIHFB2281, MGC39924
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101199
LNCipedia:   lnc-ARFGAP1-4
Entrez:   55738
Chromosome Mapping:   20q13.33
Gene Strand:   Not Available

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