PrimePCR™ lncRNA® Probe Assay:SMCR8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0135642. May amplify unspliced transcripts of an antisense gene.

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: SMCR8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: SMCR8, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0212882
Assay Design:   exonic
Chromosome Location:   chr17:18325571-18325720question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000406438
LNCipedia Splice Variants Targeted:   lnc-SMCR8-1:2 lnc-SMCR8-1:1

Gene Information

Description Not Available

Gene Symbol:   SMCR8
Gene Name:   Smith-Magenis syndrome chromosome region, candidate 8
Aliases:   FLJ34716, FLJ60657
RefSeq:   NC_000017.10 NG_017111.1 NT_010718.16
Ensembl:   ENSG00000176994
LNCipedia:   lnc-SMCR8-1
Entrez:   140775
Chromosome Mapping:   17p11.2
Gene Strand:   Not Available

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.849998
Efficiency 1.98

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