PrimePCR™ lncRNA® Probe Assay:MSC-AS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0072790

List Price:    $247.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0150030
Assay Design:   exonic
Chromosome Location:   chr8:71844403-71963013question
Amplicon Length:   81
Refseq Transcripts Amplified:   NR_033652,NR_033651
Splice Variants Targeted:   ENST00000518700 ENST00000521467 ENST00000524152 ENST00000519751 ENST00000457356 ENST00000518916 ENST00000522519 ENST00000519068
LNCipedia Splice Variants Targeted:   MSC-AS1:6 MSC-AS1:8 MSC-AS1:9 MSC-AS1:3 MSC-AS1:2 MSC-AS1:10 MSC-AS1:1 MSC-AS1:4 MSC-AS1:11 MSC-AS1:7 MSC-AS1:5

Gene Information

Not Available

Gene Symbol:   MSC-AS1
Gene Name:   MSC antisense RNA 1 [Source:HGNC Symbol;Acc:HGNC:48724]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000235531
LNCipedia:   MSC-AS1
Entrez:   100132891
Chromosome Mapping:   8q13.3
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.950001
Efficiency 1.96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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