PrimePCR™ lncRNA® Probe Assay:NCRNA00245, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0072106

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: NCRNA00245, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: NCRNA00245, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0149346
Assay Design:   exonic
Chromosome Location:   chr10:75407565-75407682question
Amplicon Length:   88
Refseq Transcripts Amplified:   NR_024421,NR_110301,NR_110299,NR_024422,NR_110300,NR_110302
Splice Variants Targeted:   ENST00000466942 ENST00000425916 ENST00000491557 ENST00000484411 ENST00000486015 ENST00000527641
LNCipedia Splice Variants Targeted:   ZNF503-AS2:12 ZNF503-AS2:13 ZNF503-AS2:3 ZNF503-AS2:2 ZNF503-AS2:7 ZNF503-AS2:6 ZNF503-AS2:9 ZNF503-AS2:8 ZNF503-AS2:10 ZNF503-AS2:11 ZNF503-AS2:5 ZNF503-AS2:4

Gene Information

Description Not Available

Gene Symbol:   NCRNA00245
Gene Name:   non-protein coding RNA 245
Aliases:   C10orf41, FLJ00096
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000237149
LNCipedia:   ZNF503-AS2
Entrez:   100131213
Chromosome Mapping:   10q22.2
Gene Strand:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.580002
Efficiency 2

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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