PrimePCR™ lncRNA SYBR® Green Assay:POU3F3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0210498. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: POU3F3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: POU3F3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0133258
Assay Design:   exonic
Chromosome Location:   chr2:104858099-104858213question
Amplicon Length:   85
Refseq Transcripts Amplified:   NM_006236
Splice Variants Targeted:   ENST00000361360
LNCipedia Splice Variants Targeted:   lnc-MRPS9-4:1

Gene Information

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al. 1996 [PubMed 8703082]).[supplied by OMIM Jul 2010]

Gene Symbol:   POU3F3
Gene Name:   POU class 3 homeobox 3
Aliases:   BRN1, OTF8
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000198914
LNCipedia:   lnc-MRPS9-4
Entrez:   5455
Chromosome Mapping:   2q12.1
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download