PrimePCR™ lncRNA SYBR® Green Assay:HMHB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0209976. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: HMHB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: HMHB1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0132736
Assay Design:   exonic
Chromosome Location:   chr5:143820484-143820615question
Amplicon Length:   102
Refseq Transcripts Amplified:   NM_021182
Splice Variants Targeted:   ENST00000289448
LNCipedia Splice Variants Targeted:   lnc-KCTD16-4:1

Gene Information

This gene encodes one of the minor histocompatibility antigens which play an important role in the induction of cytotoxic T lymphocyte (CTL) reactivity against leukemia after human histocompatibility leukocyte antigen (HLA)-identical allogeneic bone marrow transplantation (BMT). This gene is only expressed in B cell acute lymphoblastic leukemia cells and Epstein-Barr virus-transformed B cells. The translation of this mRNA initiates at a non-AUG (CUG) codon. [provided by RefSeq Jul 2008]

Gene Symbol:   HMHB1
Gene Name:   histocompatibility (minor) HB-1
Aliases:   HB-1, HLA-HB1
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000158497
LNCipedia:   lnc-KCTD16-4
Entrez:   57824
Chromosome Mapping:   5q31.3
Gene Strand:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download