PrimePCR™ lncRNA SYBR® Green Assay:DHX32, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0209932. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DHX32, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DHX32, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0132692
Assay Design:   exonic
Chromosome Location:   chr10:125852558-125859625question
Amplicon Length:   77
Refseq Transcripts Amplified:   XM_017016405,XM_017016404,NM_018180
Splice Variants Targeted:   ENST00000284690 ENST00000368721
LNCipedia Splice Variants Targeted:   lnc-UROS-3:1

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this DEAD box protein family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants but the full length nature of one of the variants has not been defined. [provided by RefSeq Jul 2008]

Gene Symbol:   DHX32
Gene Name:   DEAH (Asp-Glu-Ala-His) box polypeptide 32
Aliases:   DDX32, DHLP1, FLJ10694, FLJ10889
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000089876
LNCipedia:   lnc-UROS-3
Entrez:   55760
Chromosome Mapping:   10q26.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download