PrimePCR™ lncRNA SYBR® Green Assay:KCNH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0194194. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: KCNH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: KCNH1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0116954
Assay Design:   exonic
Chromosome Location:   chr1:210678375-210678466question
Amplicon Length:   62
Refseq Transcripts Amplified:   XM_017001246,NM_002238,NM_172362
Splice Variants Targeted:   ENST00000271751
LNCipedia Splice Variants Targeted:   lnc-KCNH1-3:1

Gene Information

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. This gene encodes a member of the potassium channel voltage-gated subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNH1
Gene Name:   potassium voltage-gated channel, subfamily H (eag-related), member 1
Aliases:   EAG, EAG1, Kv10.1, MGC142269, h-eag
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143473
LNCipedia:   lnc-KCNH1-3
Entrez:   3756
Chromosome Mapping:   1q32.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download