PrimePCR™ lncRNA SYBR® Green Assay:RHD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181720. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: RHD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: RHD, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104480
Assay Design:   exonic
Chromosome Location:   chr1:25306612-25306705question
Amplicon Length:   64
Refseq Transcripts Amplified:   XM_017002015,NM_001282872,NM_001282871,NM_016124,NM_001282870,NM_001282869,NM_001282867,XR_946736
Splice Variants Targeted:   ENST00000423810 ENST00000342055 ENST00000357542 ENST00000568195 ENST00000622561 ENST00000564398 ENST00000328664 ENST00000615607
LNCipedia Splice Variants Targeted:   lnc-TMEM50A-1:1

Gene Information

The Rh blood group system is the second most clinically significant of the blood groups second only to ABO. It is also the most polymorphic of the blood groups with variations due to deletions gene conversions and missense mutations. The Rh blood group includes this gene which encodes the RhD protein and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes and a third unrelated gene are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RHD
Gene Name:   Rh blood group, D antigen
Aliases:   CD240D, DIIIc, MGC165007, RH, RH30, RHCED, RHDVA(TT), RHDel, RHPII, RHXIII, Rh4, RhDCw, RhII, RhK562-II, RhPI
RefSeq:   NC_000001.10 NG_003255.2 NT_004610.19 NG_007494.1
Ensembl:   ENSG00000187010
LNCipedia:   lnc-TMEM50A-1
Entrez:   6007
Chromosome Mapping:   1p36.11
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download