PrimePCR™ lncRNA SYBR® Green Assay:TAF5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181119

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: TAF5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: TAF5, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103879
Assay Design:   exonic
Chromosome Location:   chr10:103388917-103389061question
Amplicon Length:   115
Refseq Transcripts Amplified:   NM_006951,NM_139052
Splice Variants Targeted:   ENST00000369839
LNCipedia Splice Variants Targeted:   lnc-TAF5-1:1

Gene Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) which binds to the core promoter to position the polymerase properly serves as the scaffold for assembly of the remainder of the transcription complex and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription serve as coactivators function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4 and it may participate in forming a nucleosome-like core in the TFIID complex. [provided by RefSeq Jul 2008]

Gene Symbol:   TAF5
Gene Name:   TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa
Aliases:   TAF2D, TAFII100
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000148835
LNCipedia:   lnc-TAF5-1
Entrez:   6877
Chromosome Mapping:   10q24-q25.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download