PrimePCR™ lncRNA SYBR® Green Assay:MRPL28, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0180517

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: MRPL28, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: MRPL28, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103277
Assay Design:   exonic
Chromosome Location:   chr16:367591-370507question
Amplicon Length:   171
Refseq Transcripts Amplified:   NM_006428,XM_011522351,XM_005255041
Splice Variants Targeted:   ENST00000483764 ENST00000461550 ENST00000469744 ENST00000481453 ENST00000199706
LNCipedia Splice Variants Targeted:   lnc-TMEM8A-2:1

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL28
Gene Name:   mitochondrial ribosomal protein L28
Aliases:   MAAT1, MGC8499, p15
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000086504
LNCipedia:   lnc-TMEM8A-2
Entrez:   10573
Chromosome Mapping:   16p13.3
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download