PrimePCR™ lncRNA SYBR® Green Assay:LINC00239, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0138450

List Price:    $169.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0061210
Assay Design:   exonic
Chromosome Location:   chr14:101731948-101732348question
Amplicon Length:   112
Refseq Transcripts Amplified:   NR_026774
Splice Variants Targeted:   ENST00000556973
LNCipedia Splice Variants Targeted:   LINC00239:3 LINC00239:2 LINC00239:1

Gene Information

Not Available

Gene Symbol:   LINC00239
Gene Name:   long intergenic non-protein coding RNA 239 [Source:HGNC Symbol;Acc:HGNC:20119]
Aliases:   C14orf72, NCRNA00239
RefSeq:   Not Available
Ensembl:   ENSG00000258512
LNCipedia:   LINC00239
Entrez:   145200
Chromosome Mapping:   14q32.31
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.400002
Efficiency 1.98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download