PrimePCR™ Probe Assay: TMEM174, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCJP0034595
Assay Design:   Exon-exon junction
Chromosome Location:   5:72469618-72469905question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000296776

Gene Information

Description Not Available

Gene Symbol:   TMEM174
Gene Name:   transmembrane protein 174
Aliases:   FLJ31268, MGC13034
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000164325
Entrez:   134288
UniGene:   Hs.508588
Chromosome Mapping:   5q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.210000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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