This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCJP0034593
PrimePCR™ PreAmp for SYBR® Green Assay: CRIP1, Human
PrimePCR™ Template for SYBR® Green Assay: CRIP1, Human
Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876) rhombotin-1 (RBTN1; MIM 186921) rhombotin-2 (RBTN2; MIM 180385) and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins 1991 [PubMed 1946385]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.