PrimePCR™ Probe Assay: ST7L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ST7L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST7L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0058500
Assay Design:   Intron-spanning
Chromosome Location:   1:113126678-113134199question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000358039 ENST00000360743 ENST00000369669 ENST00000490067 ENST00000343210 ENST00000369666 ENST00000418497 ENST00000369664 ENST00000369668 ENST00000538187 ENST00000543570

Gene Information

This gene was identified by its similarity to the ST7 tumor suppressor gene found in the chromosome 7q31 region. This gene is clustered in a tail-to-tail manner with the WNT2B gene in a chromosomal region known to be deleted and rearranged in a variety of cancers. Several transcript variants encoding many different isoforms have been described but some have not been fully characterized. [provided by RefSeq Feb 2011]

Gene Symbol:   ST7L
Gene Name:   suppression of tumorigenicity 7 like
Aliases:   FAM4B, FLJ11657, FLJ20284, ST7R, STLR
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000007341
Entrez:   54879
Chromosome Mapping:   1p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.550000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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