PrimePCR™ Probe Assay: PARVG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PARVG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PARVG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0057886
Assay Design:   Intron-spanning
Chromosome Location:   22:44577743-44579252question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000444313 ENST00000416291 ENST00000417767 ENST00000422871 ENST00000415224 ENST00000377298 ENST00000435891 ENST00000361311 ENST00000377288

Gene Information

Members of the parvin family including PARVG are actin-binding proteins associated with focal contacts.[supplied by OMIM Aug 2004]

Gene Symbol:   PARVG
Gene Name:   parvin, gamma
Aliases:   Not Available
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000138964
Entrez:   64098
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.760000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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