PrimePCR™ Probe Assay: TBC1D3C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TBC1D3C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TBC1D3C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041865
Assay Design:   Intron-spanning
Chromosome Location:   17:34581879-34583009question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000327979 ENST00000535356 ENST00000045083 ENST00000574788 ENST00000250066 ENST00000332776 ENST00000304328 ENST00000537866 ENST00000454519 ENST00000398801 ENST00000336331 ENST00000457979 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000330458 ENST00000535592 ENST00000535805 ENST00000430723 ENST00000327454 ENST00000505415 ENST00000378174 ENST00000539424 ENST00000518551 ENST00000354664 ENST

Gene Information

This gene represents one of at least 8 paralogs of TBC1D3 gene found on chromosome 17. These paralogs appear to be differentially expressed in human tissues. The proteins encoded by this gene family contain a TBC (Tre-2 Bub2p and Cdc16p) domain which is found in proteins involved in RAB GTPase signaling and vesicle trafficking. [provided by RefSeq May 2011]

Gene Symbol:   TBC1D3C
Gene Name:   TBC1 domain family, member 3C
Aliases:   FLJ78248, MGC44903, TBC1D3D
RefSeq:   NC_000017.10 NG_004113.1 NT_010799.15 NW_003315949.1
Ensembl:   ENSG00000234972
Entrez:   414060
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.290000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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