PrimePCR™ Probe Assay: OPN1LW, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: OPN1LW, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OPN1LW, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041817
Assay Design:   Intron-spanning
Chromosome Location:   X:153418530-153420141question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000367505 ENST00000373233 ENST00000369951 ENST00000442922 ENST00000334956 ENST00000550549 ENST00000369935 ENST00000430054 ENST00000309447 ENST00000369929 ENST00000430419 ENST00000259154 ENST00000597195 ENST00000595220 ENST00000595290 ENST00000596998 ENST00000599007 ENST00000594463 ENST00000599405

Gene Information

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called red cone photopigment or long-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains an N-terminal extracellular domain and a C-terminal cytoplasmic domain. This gene and the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of partial protanopic colorblindness. [provided by RefSeq Jul 2008]

Gene Symbol:   OPN1LW
Gene Name:   opsin 1 (cone pigments), long-wave-sensitive
Aliases:   CBBM, CBP, COD5, RCP, ROP
RefSeq:   NC_000023.10 NG_009105.1 NT_167198.1
Ensembl:   ENSG00000102076
Entrez:   5956
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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