PrimePCR™ Probe Assay: GAGE12H, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041802
Assay Design:   Intron-spanning
Chromosome Location:   X:49344586-49345740question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000373563 ENST00000409480 ENST00000409547 ENST00000409196 ENST00000409451 ENST00000373566 ENST00000442437 ENST00000381751 ENST00000402590 ENST00000404720 ENST00000415752 ENST00000381708 ENST00000381725 ENST00000420398 ENST00000405679 ENST00000381698 ENST00000440137 ENST00000445148 ENST00000381722 ENST00000362097 ENST00000381709 ENST00000162330 ENST00000393422 ENST00000535626 ENST00000538440 ENST00000418647 ENST00000542031 ENST00000420641 ENST00000393420 ENST00000546196 ENST00000369360 ENST

Gene Information

Description Not Available

Gene Symbol:   GAGE12H
Gene Name:   G antigen 12H
Aliases:   RP11-959H17.7
RefSeq:   NC_000023.10 NT_086939.3
Ensembl:   ENSG00000224902
Entrez:   729442
UniGene:   Hs.632813
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.670000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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