PrimePCR™ Probe Assay: POM121, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041692
Assay Design:   Intron-spanning
Chromosome Location:   7:72411467-72412419question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000409719 ENST00000567020 ENST00000293970 ENST00000564879 ENST00000299824 ENST00000438192 ENST00000373331 ENST00000305799 ENST00000367342 ENST00000413687 ENST00000367267 ENST00000367268 ENST00000395270 ENST00000358357 ENST00000434423 ENST00000446813 ENST00000257622 ENST00000257665 ENST00000453279 ENST00000307142 ENST00000429713 ENST00000434046 ENST00000379558 ENST00000403861 ENST00000329255 ENST00000220062 ENST00000421977 ENST00000409105 ENST00000409913 ENST00000319466 ENST00000409800 ENST

Gene Information

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and multiple pseudogenes have been identified. [provided by RefSeq Mar 2012]

Gene Symbol:   POM121
Gene Name:   POM121 membrane glycoprotein
Aliases:   DKFZp586G1822, DKFZp586P2220, FLJ41820, KIAA0618, MGC3792, POM121A
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000196313
Entrez:   9883
UniGene:   Hs.655217
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.820000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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