PrimePCR™ Probe Assay: MCART6, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041678
Assay Design:   Intron-spanning
Chromosome Location:   X:103349885-103401634question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000265713 ENST00000406337 ENST00000396930 ENST00000375442 ENST00000579390 ENST00000602122 ENST00000354900 ENST00000542474 ENST00000275560 ENST00000357421 ENST00000355387 ENST00000592951 ENST00000598656 ENST00000601325 ENST00000594199 ENST00000585924

Gene Information

Description Not Available

Gene Symbol:   MCART6
Gene Name:   mitochondrial carrier triple repeat 6
Aliases:   DKFZp686O1267
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000176274
Entrez:   401612
UniGene:   Hs.660022
Chromosome Mapping:   Xq22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.070000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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