PrimePCR™ Probe Assay: LYZL2, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041655
Assay Design:   Intron-spanning
Chromosome Location:   10:30900861-30915065question
Amplicon Length:   174
Splice Variants Targeted:   ENST00000336301 ENST00000582970 ENST00000508628 ENST00000334049 ENST00000223026 ENST00000520547 ENST00000441788 ENST00000315208 ENST00000537538 ENST00000435159 ENST00000397938 ENST00000406548 ENST00000331029 ENST00000414183 ENST00000332035 ENST00000360091 ENST00000332050 ENST00000361971 ENST00000538966 ENST00000400897 ENST00000445907 ENST00000251089 ENST00000305242 ENST00000375500 ENST00000277165 ENST00000333936 ENST00000340893 ENST00000375318 ENST00000392027 ENST00000295453 ENST00000295463 ENST

Gene Information

Lysozymes (see LYZ; MIM 153450) especially C-type lysozymes are well-recognized bacteriolytic factors widely distributed in the animal kingdom and play a mainly protective role in host defense. LYZL2 is a member of a family of lysozyme-like genes (Zhang et al. 2005 [PubMed 16014814]).[supplied by OMIM Apr 2009]

Gene Symbol:   LYZL2
Gene Name:   lysozyme-like 2
Aliases:   Not Available
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000151033
Entrez:   119180
UniGene:   Hs.522610
Chromosome Mapping:   10p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.940000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download