This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: POMC, Human
PrimePCR™ Template for Probe Assay: POMC, Human
This gene encodes a polypeptide hormone precursor that undergoes extensive tissue-specific post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and depending on tissue type and the available convertases processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin essential for normal steroidogenesis and the maintenance of normal adrenal weight and lipotropin beta are the major end products. In other tissues including the hypothalamus placenta and epithelium all cleavage sites may be used giving rise to peptides with roles in pain and energy homeostasis melanocyte stimulation and immune modulation. These include several distinct melanotropins lipotropins and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity adrenal insufficiency and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.