PrimePCR™ Probe Assay: FAHD2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAHD2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAHD2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041642
Assay Design:   Intron-spanning
Chromosome Location:   2:96071480-96072757question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000534358 ENST00000531904 ENST00000389506 ENST00000354520 ENST00000325888 ENST00000346177 ENST00000341552 ENST00000409865 ENST00000410037 ENST00000457968 ENST00000441968 ENST00000453220 ENST00000440566 ENST00000272610 ENST00000414820 ENST00000447036 ENST00000233379 ENST00000418606 ENST00000595462 ENST00000593514 ENST00000601525 ENST00000594623

Gene Information

Description Not Available

Gene Symbol:   FAHD2A
Gene Name:   fumarylacetoacetate hydrolase domain containing 2A
Aliases:   MGC131995
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115042
Entrez:   51011
Chromosome Mapping:   2p24.3-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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