PrimePCR™ Probe Assay: UGT2B11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: UGT2B11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UGT2B11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041531
Assay Design:   Intron-spanning
Chromosome Location:   4:70078295-70079785question
Amplicon Length:   181
Splice Variants Targeted:   ENST00000551239 ENST00000549514 ENST00000305231 ENST00000508661 ENST00000446444 ENST00000335568 ENST00000511240 ENST00000512583 ENST00000305107 ENST00000381096 ENST00000520381 ENST00000262215 ENST00000518230 ENST00000338206 ENST00000265403

Gene Information

Description Not Available

Gene Symbol:   UGT2B11
Gene Name:   UDP glucuronosyltransferase 2 family, polypeptide B11
Aliases:   MGC129611, MGC129612
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000213759
Entrez:   10720
Chromosome Mapping:   4q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 37.390000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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