PrimePCR™ Probe Assay: ZNF626, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041502
Assay Design:   Intron-spanning
Chromosome Location:   19:20829168-20844402question
Amplicon Length:   167
Splice Variants Targeted:   ENST00000313654 ENST00000399516 ENST00000373983 ENST00000450136 ENST00000236693 ENST00000382062 ENST00000309683 ENST00000476120 ENST00000545510 ENST00000319636 ENST00000591366 ENST00000313434 ENST00000391699 ENST00000511556 ENST00000506786 ENST00000401053 ENST00000359978 ENST00000439855 ENST00000596797 ENST00000376142 ENST00000359188 ENST00000376139 ENST00000376160 ENST00000376166 ENST00000376138 ENST00000355394 ENST00000346832 ENST00000376134 ENST00000376137 ENST00000536334 ENST00000490841 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF626
Gene Name:   zinc finger protein 626
Aliases:   MGC104224
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000188171
Entrez:   199777
UniGene:   Hs.657001
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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