PrimePCR™ Probe Assay: ZNF415, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: ZNF415, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF415, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041468
Assay Design:   Intron-spanning
Chromosome Location:   19:53619598-53625961question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000595962 ENST00000598016 ENST00000334564 ENST00000391788 ENST00000391787 ENST00000360465 ENST00000598192 ENST00000594530 ENST00000359798 ENST00000465448 ENST00000461779 ENST00000461321 ENST00000319783 ENST00000543227 ENST00000540744 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000357666 ENST00000444460 ENST00000391786 ENST00000457013 ENST00000438970 ENST00000270457 ENST00000535506 ENST00000332302 ENST00000434371 ENST00000391777 ENST00000597503 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF415
Gene Name:   zinc finger protein 415
Aliases:   FLJ11191
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000170954
Entrez:   55786
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.180000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download