PrimePCR™ Probe Assay: DHRS4, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041443
Assay Design:   Intron-spanning
Chromosome Location:   14:24423072-24424327question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000322475 ENST00000313250 ENST00000558263 ENST00000543741 ENST00000397075 ENST00000397074 ENST00000559632 ENST00000558581 ENST00000421831 ENST00000397073 ENST00000308178 ENST00000382761 ENST00000397071 ENST00000559411 ENST00000335125 ENST00000558753 ENST00000537912 ENST00000545240 ENST00000382755 ENST00000568908 ENST00000568909 ENST00000567835 ENST00000562631 ENST00000388813 ENST00000562558 ENST00000540164 ENST00000388812 ENST00000538815 ENST00000456916 ENST00000379694 ENST00000379696 ENST

Gene Information

Description Not Available

Gene Symbol:   DHRS4
Gene Name:   dehydrogenase/reductase (SDR family) member 4
Aliases:   CR, FLJ11008, NRDR, PHCR, PSCD, SCAD-SRL, SDR-SRL, SDR25C1, SDR25C2
RefSeq:   NC_000014.8 NG_023545.1 NT_026437.12
Ensembl:   ENSG00000157326
Entrez:   10901
UniGene:   Hs.528385
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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