PrimePCR™ Probe Assay: KLC1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041411
Assay Design:   Intron-spanning
Chromosome Location:   14:104142043-104143844question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000472726 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000568204 ENST00000348520 ENST00000380038 ENST00000389744 ENST00000557575 ENST00000553286 ENST00000347839 ENST00000555836 ENST00000334553 ENST00000246489 ENST00000557450 ENST00000452929 ENST00000554280 ENST00000445352 ENST00000537046 ENST00000553325 ENST00000553436 ENST00000555856 ENST00000345136 ENST00000357649 ENST00000354589 ENST00000398774 ENST

Gene Information

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles mitochondria and the Golgi complex. Thus kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2" this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   KLC1
Gene Name:   kinesin light chain 1
Aliases:   KLC, KNS2, KNS2A, MGC15245
RefSeq:   NC_000014.8 NG_011516.1 NG_012307.1 NT_026437.12
Ensembl:   ENSG00000126214
Entrez:   3831
UniGene:   Hs.20107
Chromosome Mapping:   14q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 37.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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