PrimePCR™ Probe Assay: CD70, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041410
Assay Design:   Intron-spanning
Chromosome Location:   19:6586361-6590876question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000379803 ENST00000248054 ENST00000284486 ENST00000534308 ENST00000528897 ENST00000598234 ENST00000344649 ENST00000423145 ENST00000245903

Gene Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated but not on resting T and B lymphocytes. It induces proliferation of costimulated T cells enhances the generation of cytolytic T cells and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation cytotoxic function of natural killer cells and immunoglobulin sythesis. [provided by RefSeq Jul 2008]

Gene Symbol:   CD70
Gene Name:   CD70 molecule
Aliases:   CD27L, CD27LG, TNFSF7
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000125726
Entrez:   970
UniGene:   Hs.501497
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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