PrimePCR™ Probe Assay: SEPHS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEPHS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEPHS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041370
Assay Design:   Intron-spanning
Chromosome Location:   10:13378300-13380754question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000319684 ENST00000327347 ENST00000413411 ENST00000425947 ENST00000378614 ENST00000537130 ENST00000545675

Gene Information

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq Sep 2010]

Gene Symbol:   SEPHS1
Gene Name:   selenophosphate synthetase 1
Aliases:   MGC4980, SELD, SPS, SPS1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000086475
Entrez:   22929
Chromosome Mapping:   10p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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