PrimePCR™ Probe Assay: RABL2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RABL2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RABL2B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041368
Assay Design:   Intron-spanning
Chromosome Location:   22:51214220-51216402question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000393167 ENST00000413545 ENST00000393165 ENST00000393166 ENST00000409875 ENST00000594202 ENST00000600676 ENST00000596536 ENST00000595033 ENST00000596951 ENST00000597512 ENST00000252771 ENST00000389133 ENST00000539407 ENST00000216144 ENST00000354869 ENST00000395593 ENST00000395598 ENST00000395595 ENST00000395590 ENST00000435118 ENST00000428313 ENST00000375315 ENST00000599205 ENST00000599617 ENST00000595999 ENST00000594223 ENST00000596737 ENST00000598380

Gene Information

The RABL2B protein is a member of the RAB gene family which belongs to the RAS GTPase superfamily. RABL2B is located within a subtelomeric region of 22q13.3. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   RABL2B
Gene Name:   RAB, member of RAS oncogene family-like 2B
Aliases:   FLJ93981, FLJ98216
RefSeq:   NC_000022.10 NT_011526.7
Ensembl:   ENSG00000079974
Entrez:   11158
Chromosome Mapping:   22q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.150000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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