PrimePCR™ Probe Assay: HMGB3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HMGB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HMGB3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041363
Assay Design:   Intron-spanning
Chromosome Location:   X:150154559-150155644question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000419110 ENST00000325307 ENST00000455596 ENST00000430118 ENST00000448905 ENST00000594285 ENST00000593327 ENST00000600124 ENST00000595259 ENST00000596539

Gene Information

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906) HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication nucleosome assembly and transcription (Wilke et al. 1997 [PubMed 9370291]; Nemeth et al. 2006 [PubMed 16945912]).[supplied by OMIM Mar 2008]

Gene Symbol:   HMGB3
Gene Name:   high mobility group box 3
Aliases:   HMG2A, HMG4, MGC90319
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000029993
Entrez:   3149
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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