This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: NUDT1, Human
PrimePCR™ Template for Probe Assay: NUDT1, Human
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP 8-oxo-dATP 2-hydroxy-dATP and 2-hydroxy rATP to monophosphates thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm with some in the mitochondria suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants some of which encode distinct isoforms have been identified. Additional variants have been observed but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional longer isoform (p26) has been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.