PrimePCR™ Probe Assay: NUDT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NUDT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NUDT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041316
Assay Design:   Intron-spanning
Chromosome Location:   7:2284307-2289578question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000356714 ENST00000397046 ENST00000397048 ENST00000343985 ENST00000339737 ENST00000397049

Gene Information

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates such as 8-oxo-dGTP 8-oxo-dATP 2-hydroxy-dATP and 2-hydroxy rATP to monophosphates thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm with some in the mitochondria suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants some of which encode distinct isoforms have been identified. Additional variants have been observed but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional longer isoform (p26) has been described. [provided by RefSeq Jul 2008]

Gene Symbol:   NUDT1
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 1
Aliases:   MTH1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106268
Entrez:   4521
Chromosome Mapping:   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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