PrimePCR™ Probe Assay: PGA5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041188
Assay Design:   Intron-spanning
Chromosome Location:   11:61017424-61018673question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000580994 ENST00000320362 ENST00000281513 ENST00000441750 ENST00000594671 ENST00000262960 ENST00000598800 ENST00000597849 ENST00000348261 ENST00000565831 ENST00000358590 ENST00000379589 ENST00000375581 ENST00000367338 ENST00000362061 ENST00000223210 ENST00000325558 ENST00000543125 ENST00000538258 ENST00000378149 ENST00000537932 ENST00000544899 ENST00000422676 ENST00000312403 ENST00000451616 ENST00000541528 ENST00000488633

Gene Information

Description Not Available

Gene Symbol:   PGA5
Gene Name:   pepsinogen 5, group I (pepsinogen A)
Aliases:   PGA3, PGA4
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000256713
Entrez:   5222
UniGene:   Hs.432854
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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