PrimePCR™ Probe Assay: RP11-468E2.1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041178
Assay Design:   Intron-spanning
Chromosome Location:   14:24664181-24679622question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000340908 ENST00000530271 ENST00000537687 ENST00000315939 ENST00000535572 ENST00000421673 ENST00000380816 ENST00000288168 ENST00000244061 ENST00000556387 ENST00000395386 ENST00000395390 ENST00000220847 ENST00000530611 ENST00000324852 ENST00000450945 ENST00000400962 ENST00000327757 ENST00000422425 ENST00000425042

Gene Information

Description Not Available

Gene Symbol:   RP11-468E2.1
Gene Name:   transmembrane 9 superfamily member 1 isoform a
Aliases:   HMP70, MP70
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000254692
Entrez:   10548
UniGene:   Hs.91586
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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