PrimePCR™ Probe Assay: GET4, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041151
Assay Design:   Intron-spanning
Chromosome Location:   7:925693-927052question
Amplicon Length:   177
Splice Variants Targeted:   ENST00000264731 ENST00000418709 ENST00000320472 ENST00000392460 ENST00000440651 ENST00000382063 ENST00000331343 ENST00000525842 ENST00000527478 ENST00000512593 ENST00000394833 ENST00000356736 ENST00000394035 ENST00000457641 ENST00000316937 ENST00000456769 ENST00000419841 ENST00000329015 ENST00000380894 ENST00000380880 ENST00000380881 ENST00000422223 ENST00000265857 ENST00000412734 ENST00000407192 ENST00000441491 ENST00000309276 ENST00000377424 ENST00000377414 ENST00000484798 ENST00000474145 ENST

Gene Information

Description Not Available

Gene Symbol:   GET4
Gene Name:   golgi to ER traffic protein 4 homolog (S. cerevisiae)
Aliases:   C7orf20, CEE, TRC35
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000239857
Entrez:   51608
UniGene:   Hs.107387
Chromosome Mapping:   7p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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