PrimePCR™ Probe Assay: ALKBH6, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041149
Assay Design:   Intron-spanning
Chromosome Location:   19:36502320-36503983question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000576741 ENST00000572344 ENST00000273371 ENST00000330817 ENST00000444914 ENST00000314669 ENST00000545060 ENST00000537681 ENST00000378875 ENST00000485128 ENST00000252984 ENST00000475223 ENST00000433672

Gene Information

Description Not Available

Gene Symbol:   ALKBH6
Gene Name:   alkB, alkylation repair homolog 6 (E. coli)
Aliases:   ABH6, MGC15677
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000239382
Entrez:   84964
UniGene:   Hs.71941
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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