PrimePCR™ Probe Assay: GAGE13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GAGE13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GAGE13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041145
Assay Design:   Intron-spanning
Chromosome Location:   X:49188095-49189271question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000442437 ENST00000381751 ENST00000402590 ENST00000404720 ENST00000415752 ENST00000381708 ENST00000381725 ENST00000420398 ENST00000405679 ENST00000381698 ENST00000440137 ENST00000445148 ENST00000381722 ENST00000362097 ENST00000381709 ENST00000343070 ENST00000307331 ENST00000568085 ENST00000568923 ENST00000322213 ENST00000361328 ENST00000276571 ENST00000344484 ENST00000382164 ENST00000544847 ENST00000470056 ENST00000293201 ENST00000597432 ENST00000593333 ENST00000600217 ENST00000600913 ENST

Gene Information

Description Not Available

Gene Symbol:   GAGE13
Gene Name:   G antigen 13
Aliases:   GAGE12A, MGC163246
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000237597
Entrez:   645051
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 36.980000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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