PrimePCR™ Probe Assay: AC108134.2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041131
Assay Design:   Intron-spanning
Chromosome Location:   16:3194607-3195750question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000443236 ENST00000422202 ENST00000355630 ENST00000402042 ENST00000407029 ENST00000396617 ENST00000297293 ENST00000546192 ENST00000535453 ENST00000233607 ENST00000238483 ENST00000399397 ENST00000373165 ENST00000276123 ENST00000360700 ENST00000395402 ENST00000542798 ENST00000521022 ENST00000416984 ENST00000521777 ENST00000524081 ENST00000397363 ENST00000368494 ENST00000338937 ENST00000396383 ENST00000562520 ENST00000268296 ENST00000562522 ENST00000395270 ENST00000338087 ENST00000428155 ENST

Gene Information

Description Not Available

Gene Symbol:   AC108134.2
Gene Name:   Putative caspase-14-like protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000228146
Entrez:   197350
UniGene:   Hs.434132
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.760000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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